The research assistant will be expected to manage the laboratory's wild-type and transgenic mouse colonies, set up matings and work with mice on a routine basis. They will also carry out various laboratory procedures following established protocols, including genotyping, RT-PCR, ELISA assays, flow cytometry, primary cell cultures, and immunohistochemistry. They will be expected to carefully track and record experimental results, analyze data using basic statistics, and present results.
This Research Assistant I will be responsible for:
- According to prescribed protocols and assigned schedules, performs a variety of routine laboratory and murine experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy.
- Assisting in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.
- Performing the following techniques typically required for the laboratory/study:
- Maintaining records of results and methodologies in a log of work performed.
- Maintaining equipment, lab area and supplies in a neat professional manner.
- Sterilizing and clean glassware, prepare media and other materials using appropriate procedures.
- Inventories supplies alerts supervisor to needed supplies, or mixes standard reagents.
- Processing order for supplies and/or animals.
- Attending courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials.
- Helping maintain murine colonies and assists in murine experiments.
- Carrying out all laboratory activities in compliance with applicable regulations and protocols.
- Conducting literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.
- Attending courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.
- Contributing to the team within and between departments and organizations.
To qualify, you must have:
- Bachelor's Degree required in STEM
- The Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles.
- The Ability to communicate effectively both orally and in writing.
- Experience with the following experimental techniques is desirable but not required: PCR, Cell culture, Flow cytometry, ELISA assays, Immunohistochemistry, Western Blots
Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.
Boston Children's Hospital offers competitive compensation and unmatched benefits, including a flexible schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, and discounted rates on T-passes (50% off). Discover your best.
Boston Children's Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.
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