| The Daley laboratory (https://daley.med.harvard.edu), a large multinational laboratory at Boston Children's Hospital, is seeking a highly motivated, responsible, conscientious, and detail-oriented individual to join our ongoing efforts to elucidate the molecular mechanisms governing stem cell activation, blood development and malignant transformation. The successful candidate must be a quick learner who enjoys acquiring new skills and working as a highly collaborative team. In addition to providing technical research support to lab members, the position will also include maintaining our mouse colonies, managing all animal health-related issues, ensuring compliance with animal regulations, and being the contact person between the Daley lab and the animal research personnel at Boston Children's Hospital.
This is a great opportunity for a current research laboratory assistant, with a high level of experience with mouse work, who is interested in assisting with experimental procedures and having a management role on all mouse related issues.
Anticipated start date is February 2021
This Research Assistant will be responsible for: - Maintain accurate, detailed, and up-to-date records concerning mouse colonies and manage replacement of breeding animals, including breeding, weaning pups, and genotyping, to ensure propagation of the lines.
- Ensure compliance with institutional and federal guidelines in health, safety and animal regulations.
- Manage all animal related issues and be the contact person between the Daley lab and the animal research personnel at Boston Children's Hospital.
- Assist with experimental procedures involving mice, including irradiation, cell transplantation, injection of compounds, as well as routine monitoring for health and surgical procedures.
- Isolate cells and tissues from experimental animals, and prepare these cells for subsequent analysis by flow cytometry, molecular analysis, immunohistochemistry, cell culture or transplantation.
- Culture mammalian cells using sterile techniques.
- Cellular analysis including immunohistochemistry, flow cytometry, DNA / RNA isolation, PCR, and other molecular biology techniques.
- Maintain accurate, detailed, and up-to date record regarding all procedures performed and results obtained.
- Operate instruments such as flow cytometer, automated fluorescence microscope.
- Ensure safe laboratory practices and compliance with institutional research guidelines.
To qualify, you must have: - Bachelor's Degree in Biological Sciences, Biochemistry, Chemistry, or related discipline; MA or MS in these areas will also be considered.
- Previous undergraduate or post-graduate lab experience, particularly with animal husbandry, sterile technique and mammalian cell culture.
- Willingness to work with animals, as well as animal and human tissues.
- Strong organizational, management and communication skills, and an ability to recognize and prioritize responsibilities.
- Ability to work hard -independently as well as with others as a team in a productive manner.
- Willingness to commit to two years.
During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs
BCH offers competitive compensation and unmatched benefits, including a rotating days/evenings and week-end schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, and discounted rates on T-passes (50% off)
BCH is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability |
