Dr. Kean's research aims to understand mechanisms underlying immune tolerance-induction in the prevention and treatment of allo-and auto-immune diseases, with projects focused on graft-versus-host disease (GvHD), cellular therapy, T cell mediators of protective immunity, and the mechanisms underlying auto-immune diseases of the GI tract. Dr. Kean's work has led to the creation of several innovative non-human primate models of T cell-mediated immune pathology and to several first-in-disease clinical trials. She has used both the animal models and the mechanistic studies linked to her clinical trials to investigate the cellular and molecular mechanisms of immune reconstitution after stem cell transplantation, graft rejection and GvHD, and to test the impact that novel immunosuppressive therapies can make on these processes.
We are looking for a highly motivated candidate to join our bone marrow transplant research using non-human primate and xenogeneic human-to-mouse models. Successful candidate will have meticulous organizational skills, attention to detail, strong work ethics, and ability to learn quickly. The position will work with other team members to support ongoing projects lead by postdocs and junior faculty. Responsibilities include: Tissue and blood sample processing, Managing biorepository and lab supply inventory, Flow cytometry, Cell sorting, nucleic acid extraction and Quantification, and general lab maintenance. Job title and compensation will commensurate with candidate's qualification and experience.
Responsibilities: - Process blood and tissue biopsy samples
- Perform multicolor flow cytometry and cell sorting
- Nucleic acid isolation and quantification
- Following specific protocols to construct bulk and single cell cDNA library for NextGen sequencing
- Perform CRISPR/Cas9-based gene editing of primary human and animal cells and cell lines
- Designing protocols for PCR- and sequencing-based assessment of gene editing efficiency
- Manage biorepository and lab supply inventory
- Ensure maintenance of reagents and supplies, and maintain adequate stocks of laboratory items
- Maintaining mouse colonies and performing stem cell transplantation experiments in mice.
In order to qualify you must have: - The knowledge of theories, principles, and concepts acquired through completion of a Bachelor of Science degree AND minimum one year of relevant experience (to qualify for Research assistant II)
- The knowledge of theories, principles, and concepts acquired through completion of a Bachelor of Science degree AND minimum three years of relevant experience OR Masters of Science degree AND one year of relevant work experience (to qualify for Research assistant III).
Preferred: - Knowledge of molecular biology, immunology, biochemistry, cell biology, or related fields - Knowledge of safe handling protocols for hazardous materials
- Experience working with lab animal (e.g. maintaining mouse colonies) is a plus
- Experience in sample processing, flow cytometry, or molecular biology techniques and procedures - Strong organization skills, problem-solving skills, and detail oriented
- Able to work with flexible hours, sometimes in the late afternoon and evening as needed
- Strong communication and interpersonal skills, able to work collaboratively in a team environment
Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.
Boston Children's Hospital offers competitive compensation and unmatched benefits.
Boston Children's Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.
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